Inclusion complex of 7-isopropoxy-isoflavon formed with cyclodextrin, the preparation thereof and pharmaceutical compositions containing these compounds as active ingredients

ABSTRACT

The invention relates to the inclusion complexes of 7-isopropoxy-isoflavon formed with cyclodextrin or cyclodextrin derivatives as well as to the pharmaceutical compositions containing these inclusion complexes as active ingredient. The invention further relates to the preparation of the new inclusion complexes. 
     The new inclusion complex according to the invention shows higher dissolution and resorption properties as compared to the active ingredient administered per se.

This is a continuation of co-pending application Ser. No. 07/273,234 filed on Nov. 17, 1988, abandoned, which is a continuation of Ser. No. 905,837 filed Sept. 10, 1986, now U.S. Pat. No. 4,826,963.

The invention relates to inclusion complex of 7-isopropoxy-isoflavon formed with any kind of cyclodextrin. In the inclusion complex according to the invention α-, β- or γ-cyclodextrin, heptakis-2,6-O-dimethyl-or heptakis-2,3,6-tri-O-methyl-β-cyclodextrin or any water-soluble β-cyclodextrin polymer having a molecular weight of about 10,000, can be used as cyclodextrin derivate.

Further, the invention relates to pharmaceutical composition containing the inclusion complex of 7-isopropoxy-isoflavon formed with any cyclodextrin derivative as active ingredient.

From the orally administred pharmaceutical composition according to the invention Ipriflavon (7-isopropoxy-isoflavon) is resorbed in pharmacologically active concentration being in not metabolized form and it can be detected in the blood.

The preparation of the Ipriflavon is described in the GB Patent Specification No. 1.360.461 and the characteristics of the compound are the following: molecular weight: 280.3; melting point: 112°-118° C.; solubility in water at 25° C.: 1-2 μg/ml. It is poorly soluble in acetone but dissolves well in chloroform and in dimethyl formamide.

The known tablet Yambolap containing 200 mg of Ipriflavon as active ingredient is used for the treatment of osteroporosis and osteomalacia. However, due to its bad solubility and resorption the therapeutical effects thereof is not sufficient.

Administering the radioactive labelled Ipriflavon to beagle dogs and detecting the amount of the radioactive material discharged, it has been observed that only the 12% of the radioactive material administered are discharged with the urine. The HPLC analysis of the blood proved that the metabolites thereof (7-hydroxy-isoflavon as main metabolite) appear in the blood.

Different efforts have been made to increase the resorption of Ipriflavon and the main metabolite (7-hydroxy-isoflavon) thereof. In the European Patent Specification No. 0129893 it is disclosed that the resorption and solubility of pharmaceutical compositions having poor solubility increase if they are ground together with inert carriers. For example 2 g of Ipriflavon were ground for 10-60 minutes together with 2 g of aerosil or 2 g of active carbon or 2 g of activated clay or 2 g of activated alumina in vibrating mill using steel balls. The solubility of the product obtained e.g. with aerosil was 20.4 μg/ml in 50% aqueous methanol after 30 minutes at 37° C., while the solubility of the unground mixture was only 15.0 μg/ml, accordingly a 1.36-fold increase of the in vitro dissolution was achieved. Analysing the blood-levels by dogs after oral administration, Ipriflavon cannot be detected in the blood. The grinding together with aerosil increases the resorption of the main metabolite from 0.152 μg.hr/ml to 0.485 μg.hr/ml which corresponds to a 3.19-fold increase.

It is well-known that that the different instable and volatile active ingredients of the different pharmaceutical compositions and pesticides become stable and crystalline when forming "molecular-capsules" with cyclodextrin. From pharmaceutical point of view the most important effect is that the inclusion complexes of the different active ingredients with poor solubility in water, become wettable with water, they can easily be dispersed and so they dissolve in water easily. Their solubility and resorption is generally 1.3-3-fold higher than that of the free, not-complexed active ingredients (W. F. Smolen and L. A. Ball: Controlled drug bioavailability, Vol. 3., page 365, John Wiley, New York, 1985).

Surprisingly we found that after administering the inclusion complex according to the invention, the in vitro solubility of Ipriflavon is 10-fold and the blood-level of the main metabolite is 15-20-fold higher as compared to that of the Ipriflavon administered per se. The resorption of Ipriflavon in an unchanged form and in pharmaceutically effective concentration can be realized as well.

The cyclodextrins are prepared from starches using cyclodextrin-glucosil transferase enzyme. There exists three different kinds of cyclodextrin, i.e. the α-, β- and γ-cyclodextrin which consists of 6,7 or 8 glucopiranose units connected with α-1,4 glucosidic bonds. The three cyclodextrine differ from each other in molecular weight, water-solubility and in hollow-diameter, accordingly they are able to form inclusion complex with the most different kinds of compounds but the inclusion complex of the same compound formed with different kinds of cyclodextrin have very different properties. There is a possibility to carry out further modifications in the cyclodextrin molecule with suitable substitutions. For example in case of dimethyl-β-cyclodextrin (DIMEB) two hydroxyl groups of every glucose unit are methylated, while in case of trimethyl-cyclodextrin (TRIMEB) all hydroxy groups are substituted by methoxy. The solubility of these compounds is even more better and their complex-forming capacity also differs from that of the unsubstituted cyclodextrin.

It is generally characteristic for the cyclodextrin that the external surface of the molecule is hydrophylic but the internal surface is apolaric and due to the size of the external spaces it is capable to include--to take in--the suitable "guest molecule". The so-called "molecular capsulating" can be realized in this way.

One of the evidence of the formation of inclusion complex between Ipriflavon and cyclodextrin is that the cyclodextrin increases the solubility of Ipriflavon in aqueous solutions. In order to determine this property, 10 mg of Ipriflavon, cyclodextrin in different amounts and 5 ml of distilled water were charged into 10 ml test tubes. After closing, the tubes were agitated for 4 days at 25° C. at 325 rpm, thereafter the samples were filtered on a G4 glass-filter and the filtrates were photometrically analysed in 50% aqueous ethanol solution. The results are shown in Table I.

                  TABLE I                                                          ______________________________________                                         Solubility of Ipriflavon in μg/ml determined                                at 25° C. in different cyclodextrin solutions                           of different concentration                                                     Cyclo-                                                                         dextrin                                                                              α- β-                                                                               γ-                                                 mg/ml CD       CD     CD     DIMEB  TRIMEB                                     ______________________________________                                          1             2.52                                                             3             7.03          22.0                                               5             11.44         40.6                                               7             15.93         59.1                                               9             19.51         77.5                                              11    4.12     15.22  2.02   96.2   8.23                                       13    4.68     15.48  2.05   114.8  9.88                                       15    5.08     15.75  2.16   191.3  11.32                                      20    6.10     13.46  2.38   284.4  14.71                                      30    8.19     7.59   2.74   416.8  26.82                                      40    9.63     6.20   3.26   588.2  43.91                                      50    10.61    5.44   3.04   761.0  61.3                                       70    14.77    6.08   3.64   1118   96.2                                       90    18.24    6.93   4.51   1142   127.5                                      100   21.63    6.64   4.76   1654   179.0                                      110   19.02    5.98   5.12   1824   218.8                                      120   23.65    5.66   5.48   2012   269.3                                      150                          2024   435.6                                      ______________________________________                                    

The solubility of Ipriflavon in distilled water is 1-2 μg/ml at 25° C. As it is evident from the data of Table I the solubility of Ipriflavon can increase in β-cyclodextrin solution--depending on the concentration of the β-cyclodextrin--up to the 15-fold of the solubility measured in distilled water. Increasing the β-cyclodextrin concentration up to 1%, the solubility of the Ipriflavon increases about up to 16-20 μg/ml but upon further increasing the cyclodextrin concentration the solubility value remains first constant then it begins to decrease when the cyclodextrin concentration exceeds the value of 1.8%. The solubility reaches the constant value of 6 μg/ml when the cyclodextrin concentration exceeds the 4% in the suspension. This value can be regarded as the solubility limit of the solid inclusion complex formed in the presence of the excess of Ipriflavon.

In γ-cyclodextrin solution the solubility does not increase considerably, only a 3-fold increase can be achieved.

In case of α-cyclodextrin the solubility of Ipriflavon increases up to the value of 20-22 μg/ml in a 12% solution and surprisingly the UV spectrum of Ipriflavon changes in such solution as well.

Using TRIMEB or DIMEB, a still greater increase of solubility can be observed. Increasing the concentration of cyclodextrin derivatives the increase of the solubility of Ipriflavon is almost linear. In a 15% solution of TRIMEB a solubility of 440 μg/ml and in a 15% solution of DIMEB a solubility of 1700-2000 μg/ml can be achieved, corresponding to a 300-fold and a 1770-fold increases of solubility, respectivelly.

Summarizing the aforementioned results, the solubility of the Ipriflavon inclusion complex according to the invention is 4-fold greater--related to the active ingredient--than that of the Ipriflavon per se, and the resorption thereof after oral administration to rats is minimum 10-fold better as compared to the blood level measured after the administration of the free active ingredient in the same dose. The unchanged 7-isopropoxy-isoflavon-level is at least 25% of the total resorptionable material.

The inclusion complex according to the invention can be prepared when 7-isopropoxy-isoflavon is reacted with any cyclodextrin derivative in aqueous-organic solvent medium. As organic solvent an alcohol or ketone can be used. Preferably, the aqueous ethanol solutions of the components are reacted--preferably at 50% ethanol concentration--then the crystalline product obtained is isolated by cooling.

According to an alternative method, the two components can be kneaded in aqueous acetone medium simultaneously with the evaporation of the solvent. Using DIMEB or TRIMEB or soluble cyclodextrin polymer, the solid, powder-like product can be isolated from the reaction mixture by evaporation, spray-drying, freeze-drying or in case of DIMEB by heating the solution.

In the following non-limiting Examples the invention is illustrated in detail.

Examples for the preparation of cyclodextrin-Ipriflavon inclusion complexes:

EXAMPLE 1 Preparation of Ipriflavon-β-cyclodextrin inclusion complex having a molar ratio of 1:2

11.0 g (39.2 mmoles) of Ipriflavon and 114.3 g (88.3 mmoles) of β-cyclodextrin of 12.36% moisture content, were dissolved in 2.25 liters of 50% by volume aqueous ethanol solution at 80° C. under vigorous stirring and left to cool to room temperature. The crystalline product precipitated was filtered and dried for 24 hours at 60° C. to give 96.4 g of product in form of loose, white powder. The particle size is smaller than 90 μm. Ipriflavon content: 10.8% by weight; Ipriflavon: -β-cyclodextrin ratio=1:2; Yield related to the active ingredient: 94.7%.

To prove that the above product is really an inclusion complex, different thermoanalytical, X-ray diffraction and dissolution analysises have been carried out.

The thermoanalitical behaviours of the mechanical mixture and the free components are almost the same, but changes in the complex due to heating can only be observed simultaneously with the decomposition of the cyclodextrin. Testing the mechanical mixture with differential scanning calorimetry (DSC) at 115° C. an endothermic peak appears corresponding to the melting point of Ipriflavon, but no peak can be observed in case of the complex. The mechanical mixture show an continuous mass-loss--according to their Ipriflavon content--between 130° C. and 250° C., but in case of complexes no mass-loss or releasing of organic substance can be observed in the same temperature interval (Thermal Evolution Analysis, TEA).

The X-ray diffraction powder diagram of the mechanical mixture consists of the additive sum of the powder diagrams of the components. However, the powder diagram of the complex contains less peaks and new peaks (2 θ°=6.7; 7.8; 20.9) being characteristic for the complex and different from the characteristic peaks of the β-cyclodextrin (2 θ°=4.5; 10.6; 12.3) and Ipriflavon appear (2 θ°=5.8; 11.5; 15.5; 17.3; 22.0), proving a new crystalline structure and so verifying indirectly the formation of the inclusion complex.

To carry out the dissolution tests 250 ml of distilled water were charged into a 500 ml flask and heated to 37° C. Thereafter the product in an amount corresponding to 50 mg of active ingredient was added then the suspension thus obtained was stirred at 1000 rpm using a magnetic stirrer. At fixed intervals 2-6 ml of samples were taken and filtered on glass-filter and after diluting, the filtrates were measured photometrically in an 1:1 mixture of water:ethanol.

The results obtained are summarized in Table II.

                  TABLE II                                                         ______________________________________                                         Dissolution of Ipriflavon, Ipriflavon-β-cyclo-                            dextrin inclusion complex preapred according                                   to Example 1 and mixture of Ipriflavon and β-                             cyclodextrin in 250 ml of distilled water at                                   37° C.                                                                  Concentration of dissolved Ipriflavon μg/ml                                                                 Ipriflavon                                                       Ipriflavon-β-cyclo-                                                                     (50.5 mg) +                                    Time  Ipriflavon  dextrin inclusion                                                                            cyclodextrin                                   (min) 51.5 mg     complex 471.5 mg                                                                             (444.7 mg)                                     ______________________________________                                          1    0.435       13.21         0.442                                           2    0.327       9.26          0.462                                           3    0.401       8.03          0.726                                           5    0.507       7.36          1.20                                           10    0.882       7.62          2.28                                           15    1.123       7.43          3.21                                           20    1.197       7.89          4.69                                           30    1.423       6.89          5.86                                           40    1.508       7.11          6.07                                           60    1.621       6.81          6.27                                           90    1.525       6.89          6.34                                           120   1.482       6.94          6.37                                           ______________________________________                                    

The dissolution of the free active ingredient is relative slow, it reaches the saturation value of Ipriflavon (about 1.5 μg/ml) after 40-50 minutes. In case of samples containing Ipriflavon-β-cyclodextrin complex, the maximum concentration of the dissolved Ipriflavon is already obtained after 1 minute, i.e. the active ingredient dissolves at once-also a flash-dissolution procceeds-, moreover at the beginning the concentration of the active ingredient may be as high as the double of the saturation value. The dissolution of the mixture of Ipriflavon and β-cyclodextrin is slower, the concentration of the dissolved active ingredient approaches the value, determined when the inclusion complex was dissolved, only after 30-40 minutes.

The dissolution of the active ingredient have been determined at different pH value as well. The dissolution curves obtained at pH=1.3 (it corresponds to the gastric acidity; HCl) and at pH=7.6 (it corresponds to the intestinal pH value; phosphate puffer) do not differ significantly from the dissolution values obtained in distilled water, accordingly the dissolution of the active ingredient is independent from the pH in the tested pH range.

EXAMPLE 2 Preparation of Ipriflavon-γ-cyclodextrin inclusion complex having a molar ratio of 1:2

0.5 g (1.78 mmoles) of Ipriflavon were dissolved in 50 ml of 96% ethanol and the solutions thus obtained was added dropwise to the solution of 8.0 g (5.35 mmoles) of γ-cyclodextrin of 13.3% moisture content in 50 ml of distilled water, during 2 hours, then the mixture left to cool to room temperature. After a further 16-hour intensive stirring the product obtained was filtered and dried for 24 hours at 60° C. to give 5.5 g of product. Ipriflavon content: 7.9% by weight, molar ratio of Ipriflavon: γ-cyclodextrin=1:2.29. Yield related to the active ingredient: 87%. The product also contains free cyclodextrin.

EXAMPLE 3 Preparation of Ipriflavon-α-cyclodextrin inclusion complex having a molar ratio of 1:2

0.5 g (1.78 mmoles) of Ipriflavon were dissolved in 10 ml of acetone in pulg mortar and 4.0 g (3.70 mmoles) of α-cyclodextrin of 10% by weight moisture content and 2 ml of distilled water were added. The light suspension thus obtained was homogenized with a standing rubbing until the solvent was evaporated. Thereafter the product thus obtained was dried for 24 hours at 60° C. It contains 11.1% of active ingredient and also free α-cyclodextrin. Molar ratio of Ipriflavon to α-cyclodextrin=1:2.1.

Formulation examples for pharmaceutical compositions containing Ipriflavon-cyclodextrin inclusion complexes as active ingredients and biological tests thereof

EXAMPLE 4

The dissolution properties of the known Yambolap tablet containing 200 mg of Ipriflavon (A), tablet containing 40 mg Ipriflavon in form of β-cyclodextrin inclusion complex prepared according to Example 1 (B) and tablet containing the mixture of 200 mg of Ipriflavon and 330 mg of β-cyclodextrin were compared at 37° C. in 500 ml of gastric juice using Erweka ZT-4 equipment. One piece of tablet was placed into the equipment and the amount of the dissolved material was determined spectrophotometrically at 247 nm.

The compositions of the tested tablets were the following:

    ______________________________________                                         Tablet A (known tablet Yambolap, K-020484):                                    Ipriflavon                 200 mg                                              Amylum maxdis               36 mg                                              Lactosum                    60 mg                                              PVP                         13 mg                                              Esma Spreng                 30 mg                                              Talcum                      7 mg                                               Magnesium                   4 mg                                               stearinicum                                                                                               350 mg                                              Tablet B:                                                                      Inclusion complex according to                                                                            370 mg                                              the invention containing 40 mg                                                 of Ipriflavon                                                                  PVP                         15 mg                                                                         385 mg                                              Tablet C:                                                                      Ipriflavon                 200 mg                                              β-cyclodextrin        330 mg                                              PVP                         20 mg                                                                         550 mg                                              ______________________________________                                    

The results of the dissolution tests are summarized in Table III.

                  TABLE III                                                        ______________________________________                                         Amounts of the active ingredient dissolved from                                the different tablets                                                                           Amounts of the                                                Time             dissolved active ingredient (mg)                              (min)     A             B      C                                               ______________________________________                                          2        --            0.80   --                                               5        0.21          1.05   0.27                                            10        0.45          1.10   0.50                                            15        0.58          1.15   0.60                                            30        0.56          1.15   0.65                                            60        0.61          1.30   0.75                                            120       0.60          1.30   1.00                                            180       0.62          1.40   1.15                                            ______________________________________                                    

The results show that only 0.62 mg of Ipriflavon dissolves out from the conventional tablet but in case of a tablet containing one-fifth of active ingredient in form of β-cyclodextrin inclusion complex compared to the amount of the active ingredient being in the conventional tablet, the quantity of the dissolved active ingredient doubles and the amount of the dissolved material exceeds with 30% the maximum value obtained by the conventional tablets after 2 minutes. In case of a tablet which contains the Ipriflavon and β-cyclodextrin in form of mechanical mixture, the dissolution was somewhat slower but even so, the quantity of the dissolved active ingredient was higher as compared to the conventional tablet.

To the resorption tests CFY male rats weighing 170-200 g were used. After a 16-hour fasting period, Ipriflavon in a dose of 25 mg/kg (group A), Ipriflavon-β-cyclodextrin inclusion complex prepared according to Example 1 (group B) and Ipriflavon-β-cyclodextrin mixture (group C)--both containing the β-cyclodextrin in equimolar ratio to the active ingredient--were administered, thereafter rat-food and water were given "ad libitum" to the animals.

The materials to be tested were mixed for 5 minutes in 2 ml of 1% methylcellulose solution and administered by the aid of a stomach tube. The plasma samples were collected 10, 30 minutes and 1, 2 and 4 hours after the treatment using mortal haemorrhage after transecting the Vena femoralis of the animals anasthesied with ether. The blood samples were charged into heparinized tubes, centrifuged, nutsch-filtered and stored deep-freezed, in vial until further use.

In order to collect urinary samples, after treatment the animals were placed into individual metabolism vessel and urine and faeces were separately collected for 24 hours. The urinary samples were stored deep-freezed until further use. The concentration of the unchanged Ipriflavon and its main metabolite (7-hydroxy-isoflavon) in the urinary and the plasma samples was followed by HPLC.

To carry out the HPLC, liquid chromatography of typ RP-18 and LiChrosorb reverse phase column (grain size in case of plasma sample: 10 μm and in case of urinary samples: 5 μm) were used. Flow velocity of eluent: 1.5 ml/min, wave length of UV detector: 254 nm, paper-velocity: 0.2 cm/min.

In case of plasma samples a mixture of 0.05M acetate buffer (pH=3):acetonitrile=60:40 and in case of urinary samples a mixture of acetate buffer:acetonitril=55:45 were used as eluent. The internal standard was ethanolic solution of 2-methyl-7-methoxy-4'-nitro-isoflavon (100 μg/ml) and to the calibration, solutions comprising 100 μg/ml and 10 μg/ml of 7-hydroxy-isoflavon in methanol were used.

To analyse the plasma samples, to 1 ml of plasma 1 ml acetate-buffer (pH=5) and β-glucuronidase (arylsulfonase) enzyme (100 FU) diluted to 10-fold with 10 μl of distilled water were added and incubated at 37° C. for 24 hours. After incubation, 50 μl of 2N NaOH were added and the mixture was extracted with benzene and centrifuged. The organic phase was separated, the aqueous phase was made acidic with 400 ml of 1N HCl, and after adding 50 μl of internal standard solution it was repeatedly extracted with 8 ml benzene. The organic phase was centrifuged, evaporated, the residue was dissolved in 100 μl of eluent and 50 μl of this solution were injected and measured.

Equation of the calibration curve: y=8.2x-0.08; correlation coefficient: 0.9999.

To analyse the urinary sample, to 0.5 ml of urine 0.5 ml of acetate buffer (pH=5) and 10 μl of β-glucuronidase (arylsulfatase) enzyme (1000 FU) were added and the mixture was extracted with 8 ml of benzene and centrifuged. The organic phase was separated, the aqueous phase was acidified with 400 ml of 1N HCl, and after adding 50 μl of internal standard solution it was extracted with further 8 ml of benzene. The organic phase was centrifuged, evaporated, the residue was dissolved in 100 μl of eluent, and 10 μl of this solution were injected into the liquid chromatograph.

Equation of the calibration curve: y=0.911x-0.09, correlation coefficient: 0.9995.

In Table IV the concentration values of unchanged Ipriflavon and in Table V the concentration values of the main metabolite determined in the blood of animals are shown. To group A Ipriflavon per se, to group B the inclusion complex according to the invention and to group C the mixture of Ipriflavon and β-cyclodextrin were administered.

                  TABLE IV                                                         ______________________________________                                         Plasma-level values in μg/ml of unchanged Ipriflavon                        as a function of time after p.o. administration                                of equivalent doses                                                                            B                                                                              Inclusion complex                                                                            C                                                Time  A         prepared acc. to                                                                             Mixture of Ipriflavon                            (h)   Ipriflavon                                                                               Example 1     and β-cyclodextrin                          ______________________________________                                         0.17  0         0.065         0.479                                                  0         0.055         0                                                      0         0.073                                                          0.5   0         0.468         0                                                      0         0.149         0                                                      0         0.194         0                                                1     0         0.271         0                                                      0         0.354         0                                                      0         0.293         0                                                2     0.078     0.514         0.081                                                  0         0.402         0                                                      0.095     0.214         0                                                4     0.041     0.148         0                                                      0         0.363         0                                                      0         0.054         0                                                24    0         0             0                                                      0         0             0                                                      0         0             0                                                ______________________________________                                    

                  TABLE V                                                          ______________________________________                                         Plasma-level values of μg/ml of main metabolite                             (7-hydoxy-isoflavon) as a function of time after                               p.o. administration of equivalent doses                                                        B                                                                              Inclusion complex                                                                            C                                                Time  A         prepared acc. to                                                                             Mixture of Ipriflavon                            (h)   Ipriflavon                                                                               Example 1     and β-cyclodextrin                          ______________________________________                                         0.17  0.156     --            2.080                                                  0.265     2.450         0.457                                                  0.195     3.650         0.767                                            0.5   0.860     12.270        0.304                                                  0.591     4.840         0.374                                                  0.365     --            0.358                                            1     0.239     12.190        0.306                                                  0.790     4.330         --                                                     0.340     3.870         0.274                                            2     1.218     22.040        0.270                                                  0.715     --            0.358                                                  1.093     3.780         0.826                                            4     0.220     1.740         --                                                     0.237     --            0.130                                                  0.235     2.520         0.330                                            24    0         0             0                                                      0         0             0                                                      0         0             0                                                ______________________________________                                    

In every period, unchanged Ipriflavon can be detected only in case of group B and it amounts to 50-500 ng/ml. In case of group A similarly to group C, unchanged Ipriflavon can be detected only in few periods (at 2 and 4 hours). As to the main metabolite in every period the plasma levels were 10-20-fold higher in group B as compared to that of groups A and C determined in the same period, i.e. the values of group B are between 3-20 μg/ml while in case of groups A and C they are between 0.2-1.0 μg/ml. In all cases a second peak appears in the plasma curve at the 2-hour-period which may be the result of an intensive enterohepatitic cycle and a more better resorption from the small intestine.

In Table VI the results of the HPLC analysis of urinary samples are shown. In the 0-24 hour urinary samples no unchanged Ipriflavon can be detected. However, the main metabolite (in all cases) can be followed. The average value of 7-hydroxy-isoflavon were 952 μg corresponding to a 3-fold increase as compared to the other groups. According to our experience the quantity of the main metabolite is 50-55% of the total quantity of the metabolite and so the total quantity discharged with urine is 2-fold higher than the quantity determined by us de facto. Upon this consideration in case of group B the 40%, while in case of groups A and C only 10-14% of the dose administered are discharged with the urine.

According to the aforementioned facts it can be proved that after administration of Ipriflavon β-cyclodextrin inclusion complex to rats a 10-15-fold increase of plasma-level and about 3-fold higher quantity of substance discharged with urine can be achieved as compared to the control.

                  TABLE VI                                                         ______________________________________                                         Amounts of main metabolite (7-hydroxy-isoflavon)                               discharged with urine, measured after the p.o.                                 administration of equivalent doses                                                                       Amounts                                                                        of the                                                                         material                                                           Tota1 amounts                                                                              discharged,                                                        of material in % of                                                     No. of discharged  the doses                                                   animals                                                                               μg       D %         .sup.--D %                               ______________________________________                                         A Ipriflavon                                                                            1        236.1       9.44                                                      2        344.4       13.77     11.34                                           3        270.4       10.81                                            B        4        925.4       37.01                                            Inclusion                                                                               5        978.9       39.16     38.09                                  complex                                                                        acc. to                                                                        Example 1                                                                      C        6        341.6       13.66                                            Mixture  7        494.3       19.77     13.33                                  Ipriflavon                                                                     and β-                                                                             8        163.9       6.56                                             cyclo-                                                                         dextrin                                                                        ______________________________________                                     

What we claim is:
 1. Inclusion complex of 7-isopropoxy-isoflavon formed with any kind of cyclodextrin.
 2. Inclusion complex of 7-isopropoxy-isoflavon formed with α-cyclodextrin as defined in claim
 1. 3. Inclusion complex of 7-isopropoxy-isoflavon formed with β-cyclodextrin as defined in claim
 1. 4. Inclusion complex of 7-isopropoxy-isoflavon formed with γ-cyclodextrin as defined in claim
 1. 5. Inclusion complex of 7-isopropoxy-isoflavon formed with heptakis-2,3,6-tri-O-methyl-β-cyclodextrin as defined in claim
 1. 6. Inclusion complex of 7-isopropoxy-isoflavon formed with water-soluble cyclodextrin-polymer having a molecular weight of >10.000 as defined in claim
 1. 7. Pharmaceutical composition, which comprises the inclusion complex of 7-isopropoxy-isoflavon formed with any cyclodextrin as active ingredient, together with a pharmaceutically acceptable inert carrier.
 8. A method of treating a subject having osteoporosis or osteomalacia, which comprises orally administering to said subject an effective amount of the complex of said 7-isopropoxy-isoflavone with a cyclodextrine defined in claim 1, wherein the solubility of the 7-isopropoxy-isoflavone in complexed form in water or in gastric juice is improved over the solubility of 7-isopropoxy-isoflavone administered per se, and wherein the levels of the 7-isopropoxy-isoflavone and its main metabolite in blood plasma are improved over said levels when 7-isopropoxy-isoflavone is administered per se. 